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TOF-SIMS for localization of 89Zr-Transtuzumab
Time of Flight Secondary Ion Mass Spectrometry imaging for precise localization of zirconium-labelled trastuzumab in xenograft cancer tumour tissues
by Florent Penen, Rene Raave, Annemarie Kip, Sandra Heskamp, Per Malmberg on behalf of the TRISTAN Consortium
Microchemical Journal 181 (2022) 107860. doi: 10.1016/j.microc.2022.107860
Abstract
The human epidermal growth factor receptor 2 (HER2) specific radiotracer zirconium-Desferrioxamine(DFO)-trastuzumab was visualized ex-vivo by Time of Flight Secondary Ion Mass Spectrometry (ToF-SIMS) imaging in ovarian and breast cancer xenograft tumor sections. Heterogeneous spatial distribution of [90Zr+] ions reflected the heterogeneous localization of trastuzumab, observed in parallel by immunohistochemistry staining in HER2+ tumors. Our results show that ToF-SIMS imaging is a quick and sensitive technique to image zirconium labelled biologics at microscale in tissues.
